Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.

نویسندگان

  • Xiaobo Feng
  • Xiaohua Fu
  • Bo Ling
  • Lei Wang
  • Wanqing Liao
  • Weihua Pan
  • Zhirong Yao
چکیده

Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 51 9  شماره 

صفحات  -

تاریخ انتشار 2013